BaculovirusTiteringKit

ExpressionSystems’BaculovirusTiteringKitfacilitatesaccuratedeterminationofinfectiousbaculovirustitersinlessthan24hours.Theassayisbasedondetectionofthebaculovirusgp64fusionproteinwhichisexpressedonthesurfaceofinfectedinsectcellswithinsixhoursofinfection.Thekitcontainsgp64-PEantibodyandabaculoviruscontrolofknowntiter.

Description:

Stainingofcellswithafluorescentlylabeledanti-gp64antibodyallowsforidentificationofinfectedinsectcells.Byinoculatingcultureswithaseriesoflogdilutionsofvirus,andstainingthecultures18–20hourspostinoculation,theratioofinfectedtouninfectedinsectcellscanbedeterminedbyflowcytometry.Statisticalanalysisofthepercentageofinfectedcellsinthevirusdilutionseriesenablesaccurateinfectioustiterdetermination.

Alternativeassaysthatalsoclaimresultsinunder24hours(suchasparticlecountinginstrumentsandqPCR)areindeedfastbutdonotdelivertrueinfectioustitervalues.Incontrasttotheplaqueassay,theculturingconditionsemployedfortheflowcytometricassaycloselyreflectactualculturingconditionsutilizedforexpressioncultures,resultinginamorerelevanttitervalueinafractionofthetime.Providedaccesstoaflowcytometerequippedtohandle96wellplates,theflowcytometricassayiseasilyadaptedtohighthroughputprocesses.

• ReproducIBLeandaccuratetiterresultswithin24hours
• Measuresinfectiousvirus
• SUSPensionculturemethodologysimulatesactualproductionconditions
• WorkswithanyflowcytometercapableofreADIngsamplesfrom96-wellplates
• Flowcytometricdatacollectionremovestheoperatorerrorofcountingplaques
• gp64-PEantibodycanbeusedalonetomonitorinfectionkineticsofbaculovirusininsectcellculture

Contents:

gp64-PE(R-PHYCOERYTHRIN(R-PE)-CONJUGATEDMOUSEANTI-BACULOVIRUSMONOCLONALANTIBODY)

Clone:AcV1

AntibodyComposition:MouseIgG2A

StorageBuffer:Phosphatebufferedsaline(PBS)containing0.05%BSA

and0.09%sodiumazide.

RecombinantBaculovirusControl

BestBac2.0co-transfectedwithemptypVL1393producedinSf9cells

Specification:

Mediatype	ESF921orESFAF
Platformcompatibility	Workswithinfectiousbaculovirusproducedbymostmethods
Shippingcondition	BaculovirusTiteringKitshipsoncoldpacks,gp64-PEshipsambient
Storagecondition	2-8⁰C,protectfromlight
Use-bydate	SixmonthsfromDateofManufactureforBaculovirusControl

RecommendedStorageCondition

2–8ºC

Expression Systems是一家致力于服务真核细胞表达的公司，尤其专注于为昆虫细胞表达领域提供创新性的昆虫细胞无血清悬浮培养基，昆虫杆状病毒表达载体，病毒滴度测定等配套产品和技术服务的美国公司。其产品目前已经受到国内使用者的肯定，上海磐世生物将携手Expression systems为国内生产科研企业带来其全系列产品，致力于利用昆虫细胞表达获得更多更好更纯的目标蛋白！ Insect Cell Culture Media and Reagents 昆虫细胞培养基和试剂品名货号产品说明ESF 921 Insect Cell Culture Medium, Protein Free无蛋白组分昆虫细胞悬浮培养基96-001无血清、无蛋白组分的昆虫细胞培养基；经测试适用于Sf9，Sf21，Tni PRO，High Five和果蝇S2细胞培养；符合cGMP标准。ESF AF Insect Cell Culture Medium, Animal Free无动物源性成分的昆虫细胞培养基99-300不含任何动物或动物来源成分的培养基；经测试适用于Sf9，Sf21，High Five和果蝇S2细胞培养；符合cGMP和FDA标准。Production Boost Additive-A Nutritive Supplement昆虫细胞培养补料充添加剂95-006-100PBA能提高蛋白的表达；无动物源性成分，添加入ESF 921或者ESF AF使用。921 Delta Series, Methionine Deficient甲硫氨酸缺失培养基96-200在ESF 921基础上，一系列氨基酸缺失的培养基，可用于蛋白标记和蛋白结构分析研究。921 Delta Series, Leucine Deficieng亮氨酸缺失培养基96-210921 Delta Series, Phenylalanine Deficient苯丙氨酸缺失培养基96-230921 Delta Series, Phenylalanine and Valine Deficient苯丙氨酸和缬氨酸缺失培养基96-250921 Delta Series, 7 Amino Acid Deficient异亮氨酸、亮氨酸、甲硫氨酸、苯丙氨酸、苏氨酸、缬氨酸和酪氨酸缺失培养基96-260Molecular Products DNA and Vectors 昆虫杆状病毒表达相关产品（载体和转染介质）Baculovirus Titering Kit杆状病毒滴度检测试剂盒 97-101利用AcMNPV GP64特异性抗体（偶联PE）结合流式细胞计数对病毒滴度进行快速大通量检测。BestBac 1.0, Linearized Baculovirus DNA91-001野生AcMNPV的E2克隆，用于外源蛋白表达BestBac 2.0, v-cath/chiA Deleted Linearized Baculovirus DNA91-002缺失了AcMNPV基因组中的组织蛋白酶和几丁质酶基因，在晚期外源蛋白表达过程中可提高目的蛋白的完整性，降低被降解的可能性，增加功能蛋白的产生。PEI TR Transfection Reagent95-050适用于多种细胞系的转染Expres2 TR Liposome Based Transfection Reagent脂质体转染试剂95-055基于脂质体的转染试剂Transfection Medium转染培养基95-020用于病毒载体的转染，可有效提高转染效率，增加病毒滴度及瞬时表达水平。BestBac 1.0, Baculovirus Cotransfection Kit杆状病毒表达试剂盒91-100该试剂盒包含BestBac 1.0, Baculovirus DNA， pVL1392-lacZ control Vector，Transfection Medium, Expres2TR Transfection ReagentBestBac 2.0, Baculovirus Cotransfection Kit杆状病毒表达试剂盒91-002该试剂盒包含BestBac 2.0, v-cath/chiA Deleted Baculovirus DNA， pVL1392-lacZ control Vector，Transfection Medium, Expres2TR Transfection ReagentMammalian Cell Culture Media 哺乳动物细胞培养基ESF SFM Serum-Free Medium for Hybridoma, CHO& 293 cells无血清培养基98-001适用于杂交瘤，CHO和293细胞悬浮的培养基。